DNA-based ddPCR Methods
This chapter covers a few different ddPCR methods related to the detection of multiple variants with drop-off probes, gene edits, pathogens, linkage, performing long amplicon ddPCR, MPddPCR, MSAddPCR, methylation analysis using MSREs, utilizing BDA as an approach to enrich for mutant templates in your mixed samples, running amplitude multiplexing for multiple mutation detection in addition to simplified multiplex mutation detection strategies, performing viral integration copy number estimations, detecting structural variants and distinguishing alive (from dead) cells.
Table of contents
- Allelic Imbalance for CNV
- CAR-T Therapy
- Drop-off Probes
- Gene Editing with CRISPR/Cas
- Gene linkage EHEC example
- Linkage and Phasing
- Long Amplicon (LA) ddPCR
- Mediator Probe ddPCR
- Methylation
- Mutant Enrichment via Blocker Displacement Amplification
- Mutation Detection with Amplitude Multiplexing
- Mutation-Selected Amplification ddPCR
- Quantifying Alive vs Dead Cells with ddPCR
- Simplified Multiplexing Strategies for Mutation Detection
- Single-cell Vector Copy Number Variation Analyses
- Structural Variants
- Super Selective Primer Mediated Mutant Enrichment
- Ultra-Rapid ddPCR