RNA and Protein-based ddPCR Methods

In this chapter you will find methods aimed at detecting and quantifying RNA and/or Protein molecules. Topics such as integrity of long RNA molecules, average RNA copies per target gene, single cell RNA & protein quantification, miRNA quantification, single cell mutant RNA enrichment, extracellular vesicle detection and single cell transcript profiling and radial multiplexing are covered.

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Table of contents

• Integrity of Long RNA
• Ligation Probes for miRNA Quantification
• Quantification of Extracellular Vesicles
• Selfie-dPCR
• Single Cell Mutant RNA Enrichment
• Single-cell RNA & Protein Quantification
• Single-Cell Transcript Profiling & Radial Multiplexing
• Stem Loop Primers for miRNA Quantification
• Wastewater Monitoring